NAD+ 500mg: Product Details, Quality and Ordering
NAD+ is often searched for as “NAD peptide,” but nicotinamide adenine dinucleotide is not a peptide at all. It is a dinucleotide coenzyme found in every living cell, where it carries electrons through metabolic reactions and serves as a substrate for enzymes involved in DNA repair and cellular signalling. That distinction shapes how the compound should be described, tested and handled in a laboratory.
At Peptide King, our NAD+ 500mg is listed as a lyophilized research material. The current page states a 500mg nominal quantity, molecular weight of 663.43 g/mol, CAS number 53-84-9, ≥98% HPLC-verified purity, and storage at -20°C protected from light. It is supplied exclusively for in-vitro laboratory research.
This guide explains what NAD+ is, what researchers have studied, and which quality details deserve attention before laboratory use.
What Is NAD+?
NAD+ is the oxidized form of nicotinamide adenine dinucleotide. It works in a pair with its reduced form, NADH, in the redox reactions at the centre of energy metabolism.
NAD+ Is a Coenzyme, Not a Peptide
Peptides are chains of amino acids joined by peptide bonds. NAD+ contains no amino acids.
It is built from two nucleotides, one carrying a nicotinamide group and the other an adenine group, joined through their phosphate groups. Its molecular formula is C21H27N7O14P2, and the 663.43 g/mol molecular weight on the Peptide King page is consistent with that structure.
The “NAD peptide” label is common because NAD+ is often sold beside peptides. Chemically, it belongs to a different class.
NAD+ Has Two Broad Roles in the Cell
The first is redox chemistry. NAD+ accepts electrons during glycolysis, the citric acid cycle and fatty acid oxidation, becoming NADH, which then supplies electrons to mitochondrial respiration.
The second is signalling. Several enzyme families consume NAD+ as a substrate rather than recycling it, including sirtuins, poly(ADP-ribose) polymerases (PARPs) and CD38.
What Has NAD+ Research Actually Studied?
NAD+ biology is one of the most active areas of metabolism and ageing research. Most of that work examines NAD+ levels and the enzymes that depend on them.
Sirtuins and PARPs Depend on NAD+ Availability
Sirtuins are NAD+-dependent enzymes involved in gene regulation and metabolic adaptation. PARPs use NAD+ during the cellular response to DNA damage.
Because both families consume NAD+, researchers study how NAD+ availability affects their activity in cell and animal models.
NAD+ Decline Is a Recurring Research Theme
Studies in animal models and some human tissues have reported lower NAD+ levels with age. That observation drives much of the interest in whether restoring NAD+ affects mitochondrial function, DNA repair and metabolic regulation in experimental systems.
Much Human Research Uses Precursors, Not NAD+ Itself
Many human studies of NAD+ metabolism use precursors such as nicotinamide riboside (NR) or nicotinamide mononucleotide (NMN) rather than NAD+ directly.
How cells take up extracellular NAD+ is still debated, and several studies indicate that much of it is first converted into smaller precursors. Findings from precursor studies should not be assumed to apply to NAD+ material, and the reverse is also true.
Peptide King presents NAD+ as laboratory research material, not a supplement or medical treatment.
What Does the Peptide King NAD+ 500mg Page Specify?
The live NAD+ 500mg research product page gives researchers several specifications directly.
The Listed Specifications Are Clear
The current page identifies:
- Quantity: 500mg
- Form: lyophilized powder
- Purity: ≥98% HPLC verified
- Storage: -20°C protected from light
- Molecular weight: 663.43 g/mol
- CAS number: 53-84-9
The Page Links a Certificate of Analysis
The NAD+ 500mg page links a Certificate of Analysis. The linked report states that analysis was performed by HPLC with UV detection coupled with mass spectrometry, and it reports a purity of 99.963% and a measured content of 488.79mg.
That report answers three separate questions: identity, purity and quantity.
Purity and Measured Content Are Different Results
An HPLC purity result describes the proportion of detected material in the principal chromatographic peak. It does not describe how many milligrams are in the vial.
The measured-content result answers that second question. A nominal quantity and a measured quantity are two different data points, and a complete research record keeps both.
Why Does a Certificate of Analysis Matter for NAD+?
NAD+ is a well-defined molecule, but research material still needs documentation tied to the lot being used.
NAD+ Stability Makes Batch Records More Useful
NAD+ is relatively stable as a dry powder but less stable in solution, particularly under heat or alkaline conditions. NADH is a separate compound with different properties.
That makes storage details, test dates and compound identity worth checking together.
Researchers should look for:
| CoA field | What to verify |
|---|---|
| Compound name | NAD+, not NADH or a precursor |
| Molecular weight | Consistent with 663.43 g/mol |
| Lot number | Matches current inventory |
| Purity | Method and result are reported |
| Identity | Mass spectrometry or equivalent support |
| Measured content | Reported separately from purity |
| Test date | Relevant to the current batch |
How Does NAD+ Relate to Other Metabolic Research Compounds?
NAD+ often appears beside compounds studied in related metabolic pathways.
Shared Pathways Do Not Make Compounds Interchangeable
Peptide King also lists 5-Amino-1MQ 5mg, a small-molecule NNMT inhibitor studied in connection with NAD+ salvage, and MOTS-c 20mg, a mitochondrial-derived peptide studied in metabolic regulation.
NAD+ is a coenzyme, 5-Amino-1MQ is a small molecule, and MOTS-c is a 16-amino-acid peptide. They can appear in overlapping research areas, but their structures, mechanisms and evidence differ.
How Does Canadian Fulfillment Work?
Peptide King fulfills research orders within Canada.
Domestic Processing Follows the Site’s Standard Timeline
The site states that orders are typically processed within one to two business days, with standard shipping estimated at three to seven business days depending on province. Tracking is provided after dispatch.
How We Present NAD+ 500mg at Peptide King
At Peptide King, we present NAD+ as a research compound with a deep scientific literature and a frequently misused label.
It is a coenzyme, not a peptide, and many of the best-known findings about NAD+ come from cell and animal models or from human studies of its precursors.
Researchers should confirm the compound form, separate purity from measured content, match the CoA to the lot, and keep precursor findings separate from NAD+ findings.
For research-grade NAD+ 500mg, getting the chemistry right is the first quality check.